Many varieties of TB germs is causing problem in the final diagnosis of TB in female genital organs. please be informed on that.

hindrance in Laboratory diagnosis of TB germs from tissues removed from human body. What are the different types of Mycobacteria exist in and around Humans?

Mycobacteria are a diverse group of rod-shaped bacteria that include more than 100 different species. The others, which are far commoner, environmental mycobacteria; and when the TB PCR test. But quite often contaminations occur e.g. tap water, slides etc and giving a false + ve DNA –PCR +ve. AS such, it is important to discriminate between true infection and contamination. The molecular cross-reaction between the ubiquitous non-pathogenic environmental mycobacteria (which are harmless colonizers) and M tuberculosis is what creates the diagnostic dilemma.

One decade back it was thought that the diagnostic of this diseases & prevailing dilemma has come to full stop with the advent of PCR method.

Sadly, later it was known that -The TB PCR test is highly flawed, because the DNA sequence which the PCR amplifies is common to both the mycobacterium tuberculosis as well as the other species of mycobacteria.