Thursday, 23 July 2020

Basics of subfertility


Are you treating subfertile couples: Then must know followings: What is Fecundability? : Ans:-probability of achieving pregnancy within one menstrual cycle. For a normal couple, this is approximately 25%.Q. What is Fecundity? Ans: : Ability to achieve a live birth within one menstrual cycle . Prevalence of subfertility?? Data from the 2006 to 2010 NSFG continued to show that as in the 2002 data, 11.9% of women of reproductive age reported having received infertility services at some point in their lives. The What are common  causes of subfertility? This  encompasses six  principal categories
1)                   Male factor & Coital  dysfunction 2) Ovulatory dysfunction, other endocrine disorders including hypothyroidism, hyperprolactinaemia, Adrenal disorders , hyperandrogenism, hyperinsulinaemia
3)                   Structural (tubal/peritoneal and uterine)
4)                   Endometriosis & Kochs , Pelvic adhesive disease
5) Cervical factors 6) Unexplained causes.
 Complete menstrual history, dysmenorrhea or menorrhagia, pelvic or abdomi­nal pain, dyspareunia, symptoms of thyroid disease, galactorrhea, symptoms of hirsutism, exercise habits, diet history is essential and indices of stress has to be assessed.

The causes   infertility encompasses five principal categories

.Q.1:-When to initiate investigation for fertility disorders?  Ans: By and large if woman is asymtomatic i,.e. with no systemic symptoms, she is  aged < 35 yrs  without any  menst irregularities(cycles are regular)  and is of normal BMI  then investigations should be  initiated one year  after marriage. But if the age of F partner is > 35 yrs then Fertility investigations should commence earlier
Q.2 : How many months after marriage one should initiate fertility related investigations if she is aged > 35 years? Ans: In such settings most practitioners will initiate diagnostic evaluation after inability to conceive for 6 months.
Q.3: What are the list of routine infertility evaluation? Ans Prior to investigations some enquiry is relevant. Such are :-1) elicitation of  history of any medical/ surgical diseases in past, any history of miscarriage, previous marriage if any , drug abuse, addiction, any regular drug intake for chr disease , contraceptive use ,This should be followed by  clinical examination of both partners. BMI of both partner are important so also any endocrine stigmata. Duration of infertility, methods of contraception, previous evaluation and treat­ment, prior reproductive history, sexual dysfunction, coital frequency and satisfac­tion, sexually transmitted infections, tobacco and alcohol use, caffeine use, family history of mental retardation, and birth defects
’ History elicited from the female partner should include the following:
Complete menstrual history, dysmenorrhea or menorrhagia, pelvic or abdomi­nal pain, dyspareunia, symptoms of thyroid disease, galactorrhea, symptoms of hirsutism, exercise habits, and indices of stress

  Q. 2B Basic Investigations : 1 semen analysis, , evaluation of reproductive hormones like PRL and TSH  5)basal scan &  , follow up scan in unstimulated cycle. If she denies to come to clinic frequently for evaluation in natural cycle then assessment of ovulation can be done by LH kit at home . But in doing  that we miss a) lagging of endometrial thickness, b) speed of growth of DF,c)  No of growing  follicles  and d)  possible LUF.  However a regular cycling woman means that in all probality she is ovulating. Hysterosalpingogram, and, laparoscopy are done  if indicated. Lap is very informative may be done at an early stage of evaluation if clinical findings suggest  Pelvic adhesions, or  endometriosis . Fixity of uerus andndrness of POD/ adenexae with ormal Total couny & CRP  almost usulay poiny to such diseases which can onlybe diagnosed by LH procedute Weight and body mass index, thyroid exam, breast exam, signs of hirsutism, pelvic or abdominal tenderness, uterine size and mobility, adnexal masses and/or tender­ness, cul-de-sac tenderness, or nodularity
   Baseline studies and labs may include the following: thyroid-stimulating hormone, prolactin, follicle-stimulating hormone (FSH), 17-hydroxyprogesterone, serum testosterone, progesterone, dehydroepiandrosterone (DHEAS), semen analysis, and hysterosalpingogram (HSG).

Q.3:-How to carry on “ A complete workup of unexplained infertility” . If not doe earlier  then one should insist on following tests like A) AMH, AFC estimation, Day3 basal FSH  are tests for ovarian reserve and B) laparoscopy-hysteroscopy even if HSG or SIS were normal  at initial stages of fertility evaluation before reporting to you.
Q What are the benefits  of Hysteroscopy??

initial hysteroscopy. Findings on hysterescopy (%)
Submucosal myoma
Single large polyp
Polypoid endometrium
Endometrial hyperplasia
Uterine cavity hypoplasia
Adhesions
Endocervical polyp
Multiple lesions
Without any finding

 According to the results of any studies abnormal hysteroscopic findings were observed
in about 60% of the cases with repeated IVF failures which
are higher than the result of previous studies due to the
exclusion of the other possible reasons of repeated ET
failures. It is said that  sonography as specific, but not
sensitive method compared to the hysteroscopy. The sensitivity and specificity of sonography in comparison with hysteroscopy were 91% and 83% with the false
positive rate of 9.2%, and false negative rate of 5.1%
in Ragni study.  We treated the structural abnormalities
at the time of hysteroscopy, and we had no early, late,
major, or even minor complications in our series. The
pathological reports confirmed the observed lesions in
94.7%, with nonspecific endometritis in 13.2 % (n= 7)
of cases.
It has been shown that in elderly women, age-
related uterine pathology such as submucous myoma,
endometrial hyperplasia, and polyps were more
prominent, other than in younger patients, other uterine
lesions such as adhesions, and tubal ostia occlusion
were more common.  In our experiences, we have seen
single polyp and endometrial hyperplasia more (but not
significant) in the patients with ≤30 years old rather
than the younger group (p-value= 0.78). Also there
were more (but not significant) abnormalities especially
single polyp, polypoid endometrium, and hyperplasia
in group of the patient with more than 8 years infertility
(p=0.08, p=0.06, and p=0.12).
This communication, emphasis the need of
hysteroscopy in patients with unexplained repeated
IVF/ICSI-ET failure According to our experience, it
seems legitimate to perform hysteroscopy in women
who had 2 IVF-ET failures before trying any other
procedure after excluding all other possible etiological
or accompanying factors. The results were significant in
the group with longer duration of the infertility. Our
study showed that the direct view of the uterine cavity
by hysteroscopy was more sensitive and specific, and more accurate in the evaluation of intracavity pathology
in comparison with sonography in patients with
unexplained repeated IVF failures.
 Also, hysteroscopy proved to be a very useful, accurate and safe method of assessing uterine and endometrial functional status
in patients with repeated IVF/ICSI-ET failures after excluding other possible reason for implantation failure, although it couldn’t evaluate uterine and endometrial
functional status.


Pont 1: Early assessment of Ovarian reserve; if not on first visit disregarding her age. The overall incidence of infertility has remained stable over the decades but investigative schedules , as I have witnessed for six decades, has changed considerably. Many a experienced dedicated practitioner have of late have included the evaluation of ovarian reserve as first-line workup for infertility. May be environmental pollution , increased prevalence of  poor lifestyle, diet, drug abuse and auto immune diosrder  might have collectively  forced the ART specialists to carryout Ov reserve test  at an early date .

Q.2 : Prevalence of Unexplained infertility:-Approximately 15% to 30% of couples will be diagnosed with unexplained infertility after their diagnostic workup which however do not   include Hysterolaproscopy bu does include Folicular monitoring , HSG/SIS and basal frertlity hormonres (FSH,LH, PRL ) and some sytemic hormes like TSH .
 Q. Ovulatory defects are present in 40% of infertile women and in approximately 15% of couples with infertility anovulatory levels of serum progesterone (< 3 ng/mL) are found in only a very small minority of eumenorrheic patients. We recommend that couples have a semen analysis, testing for detection of ovulation (mid luteal progesterone, LH kit), assessment of ovarian reserve, transvaginal ultrasound, and HSG. With this expanded testing, fewer than 15% to 30% of couples will have unexplained infertility.
ovulation kit and measure the progesterone levels 7 to 8 days after the LH surge is detected. Whereas laparoscopy used to be part of the basic infertility workup, it is now reserved for selected cases
Serum progesterone levels higher than 3 ng/mL suggest that ovulation has occurred and levels higher than 10 ng/mL are optimum. According to the guidelines of the ASRM, laparoscopy should be performed in women with unexplained infertility or signs and symptoms of endometriosis or in whom reversible adhesive tubal disease is suspected. The principal treatments for unexplained infertility include expectant observation with timed intercourse and lifestyle changes, clomiphene citrate and intrauterine insemination (IUI), controlled ovarian hyperstimulation (COH) with IUI, and IVF. fecundity were 4.7 and 2.4 per 100 person-months (rate ratio, 1.9; 95% confidence interval, 1.2–3.1). The authors concluded “laparoscopic resection or ablation of minimal and mild endometriosis enhances fecundity in infertile women. Reproduction and Embryology (ESHRE) Multicentre Trial reported in 1991 that pregnancy rates per cycle were 15.2% in gonadotropin-only cycles, 27.4% in gonadotropin and IUI cycles, and 25.7% in IVF cycles. The pregnancy rate for IVF cycles has since increased with availability of improved and sequential media, embryo micromanipulation, and extended embryo culture. The ASRM Practice Committee published an analysis on the appropriate roles and the cost-effectiveness of the various procedures in the management of unexplained infertility by analysis of previously published data.2,14 There is limited information on the cost-effectiveness of various treatments; however, it appears that there is a correlation between the cost of a treatment modality and its pregnancy rates. Therefore, depending on the individual couple and their particular clinical situation, COH with IUI may be attempted first, with transition to IVF/ICSI if pregnancy is not achieved in a timely manner.
Fast Track and Standard Treatment [FASTT] Trial) :--Clomiphene citrate/IUI versus IVF (Fast Track and Standard Treatment [FASTT] Trial) has recently been completed. In this trial, Reindollar and colleagues27 studied 503 couples assigned to conventional infertility treatment or an accelerated track to IVF. All couples had unexplained infertility and underwent infertility treatment for the first time. Patients were randomized to receive either a conventional treatment regimen of 3 cycles of clomiphene/IUI, 3 cycles of FSH/IUI, and up to 6 cycles of IVF or to receive an accelerated treatment course of 3 cycles of clomiphene citrate/IUI and then up to 6 cycles of IVF. In the conventional arm, 247 couples underwent 646 clomiphene citrate/IUI, 439 FSH/IUI, and 261 IVF treatment cycles; in the accelerated arm, 256 couples received 642 clomiphene citrate and 357 IVF cycles.
Outcome of Conventional arm As of April 2007, 43/232 (18.5%) of the women in the conventional arm became clinically pregnant after clomiphene citrate/IUI, 43/170 (25.3%) after FSH/IUI cycle, and 71/111 (64%) after IVF.
 Outcome of   the accelerated arm, 50/242 (20.7%) became pregnant after clomiphene citrate/IUI and 117/171 (68.4%) in an IVF cycle. The median time to pregnancy in the accelerated arm was shorter than the conventional arm. The complete study has not yet been published.






Rev Obstet Gynecol. 2008 Spring; 1(2): 69–76.
PMCID: PMC2505167
Diagnosis and Treatment of Unexplained Infertility
Alexander Quaas, MD, PhD* and Anuja Dokras, MD, PhD
This article has been cited by other articles in PMC.
Abstract
Over the past decade, significant advances have occurred in the diagnosis and treatment of reproductive disorders. In this review, we discuss the routine testing performed to diagnose unexplained infertility. We also discuss additional testing, such as assessment of ovarian reserve, and the potential role of laparoscopy in the complete workup of unexplained infertility. Finally, we outline the available therapeutic options and discuss the efficacy and the cost-effectiveness of the existing treatment modalities. The optimal treatment strategy needs to be based on individual patient characteristics such as age, treatment efficacy, side-effect profile, and cost considerations.
Key words: Infertility, unexplained; Semen analysis; Ovulation assessment; Ovarian reserve; Intrauterine insemination; Controlled ovarian hyperstimulation; In vitro fertilization
Significant advances have occurred in the diagnosis and, more importantly, in the treatment of reproductive disorders over the past decade. The overall incidence of infertility has remained stable1; however, the success rates have markedly improved with the widespread use of assisted reproductive technologies. Treatment options and success vary with the cause of infertility. Approximately 15% to 30% of couples will be diagnosed with unexplained infertility after their diagnostic workup.2 

In this review, we specifically discuss the routine testing performed to diagnose unexplained infertility. We also discuss additional testing, such as assessment of ovarian reserve, and the potential role of laparoscopy in the complete workup of unexplained infertility. Finally, we outline the available therapeutic options and discuss the efficacy and the cost-effectiveness of the existing treatment modalities.
Diagnosis of Unexplained Infertility: The Basic Infertility Evaluation
Infertility is customarily defined as the inability to conceive after 1 year of regular unprotected intercourse. The infertility evaluation is typically initiated after 1 year of trying to conceive, but in couples with advanced female age (> 35 years), most practitioners initiate diagnostic evaluation after an inability to conceive for 6 months.
The Practice Committee of the American Society for Reproductive Medicine (ASRM) has published guidelines for a standard infertility evaluation.3 It includes a semen analysis, assessment of ovulation, a hysterosalpingogram, and,
if indicated, tests for ovarian reserve and laparoscopy.
When the results of a standard infertility evaluation are normal, practitioners assign a diagnosis of unexplained infertility. Although estimates vary, the likelihood that all such test results for an infertile couple are normal (i.e, that the couple has unexplained infertility) is approximately 15% to 30%.2
Assessment of Male Infertility
Male factor infertility is the only cause of infertility in approximately 30% of couples and a contributing factor in another 20% to 30%.4 
Assessment of the infertile couple includes evaluation of the male partner by history, examination, and semen analysis. Important elements of the history include prior paternity, a history of cryptorchidism, medical and surgical history, sexual dysfunction, and any use of medications, tobacco, alcohol, or illicit drugs. On the physical examination, testicular abnormalities such as a varicocele or absence of the vas deferens can be detected.
If the semen analysis is abnormal, it should be repeated after at least 1 month by a laboratory that adheres to World Health Organization (WHO) guidelines, with a quality control program ensuring accurate testing. Guidelines from WHO regarding the reference ranges for the number, morphology, and motility in a semen sample have been published5 and are shown in Table 1.

A number of commercial laboratories use a variety of ranges for the different components of the semen analysis.
 Careful attention should be paid to these ranges and the semen values should be interpreted in the right context. Although semen analysis is routinely used to evaluate the male partner in infertile couples, the discriminatory ranges are not clearly defined. A study by Guzick and colleagues concluded “threshold values for sperm concentration, motility, and morphology can be used to classify men as subfertile, of indeterminate fertility, or fertile. None of the measures, however, are diagnostic of infertility.” Table 2 shows the reference values for fertile, indeterminate, and subfertile ranges for semen analysis parameters as identified by regression analysis. Despite its limitations, semen analysis remains the most important tool in the investigation of male factor infertility. If any abnormalities are repeatedly detected on a semen analysis, referral to a urologist may be warranted. The treatment of severe male factor infertility including azoospermia has been revolutionized with the combination of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).
World Health Organization Criteria for a Normal Semen Analysis
Fertile, Indeterminate, and Subfertile Ranges for Semen Analysis Parameters as Identified by Regression Analysis
Previously, the postcoital test (PCT) assessing the sperm motility in a sample of postcoital cervical mucus was considered an integral part of the basic infertility evaluation. However, past investigations revealed a poor correlation between postcoital sperm motility and pregnancy outcome.7 In addition, a 1995 blinded, prospective study demonstrated poor reproducibility of the test among trained observers, further questioning the validity of the PCT as a diagnostic tool.8 

Today, the PCT has been largely abandoned and we do not recommend it as a component of the standard infertility investigation.3
Assessment of Ovulation
Ovulatory defects are present in 40% of infertile women and in approximately 15% of couples with infertility.

Often a defect in ovulatory function manifests itself in menstrual disturbances and can be identified by history in the majority of women.  A) What is the history?? A patient with causes of menstrual abnormalities should be investigated for underlying causes such as 1) polycystic ovarian syndrome,2)  thyroid disease, 3) hyperprolactinemia, and 4) hypothalamic causes secondary to weight changes. Eumenorrhea—normal menstrual cycles by history—is a highly accurate marker of ovulation and Ovulatory defects are present in 40% of infertile women and in approximately 15% of couples with infertility anovulatory levels of serum progesterone (< 3 ng/mL) are found in only a very small minority of eumenorrheic patients.
In addition to a thorough menstrual history, other methods used to evaluate ovulation include basal body temperature (BBT) recordings, urinary luteinizing hormone (LH) ovulation predictor kits, mid luteal serum progesterone testing, and endometrial biopsy to assess for secretory endometrial development.  B) What about BBT?? Although BBT recordings are the least costly tool in a reliable patient (Figure 1), they are difficult to interpret and often frustrating for the patient.
 ) What about Ovulation predictor kits ? Ovulation predictor kits are useful for women who do not have very long menstrual cycles and can be used by couples to appropriately time intercourse.

) What about Mid luteal progesterone levels?? Ans:- Mid luteal progesterone levels are measured around day 21 in women with regular ( 28 day) cycles. However, they are often poorly timed if they are drawn on cycle day 21 in women with irregular menses. In such women it is better to use an ovulation kit and measure the progesterone levels 7 to 8 days after the LH surge is detected.
Serum progesterone levels higher than 3 ng/mL suggest that ovulation has occurred and levels higher than 10 ng/mL are optimum. Although endometrial biopsy results were previously used to diagnose luteal phase defect, they do not correlate with fertility status and hence are no longer recommended.10
An example of a basal body temperature recording chart. Reprinted with permission from Hyde and DeLamater, Understanding Human Sexuality, 6th ed. Copyright ©1997 The McGraw-Hill Companies, Inc. All rights reserved.
Assessment of Ovarian Reserve
Another test added to the workup of couples with infertility includes assessment of ovarian reserve. Women with advanced age or history of prior ovarian surgery are at risk for diminished ovarian function or reserve. Given the relatively noninvasive nature of the testing, several practitioners are including the evaluation of ovarian reserve as first-line workup for infertility.

The testing includes a cycle day 3 serum follicle-stimulating hormone (FSH) and estradiol level, clomiphene citrate challenge test, and/or an ultrasonographic ovarian antral follicle count.3 The results of these tests are not absolute indicators of infertility but abnormal levels correlate with decreased response to ovulation induction medications and lowered live birth rates after IVF.
Assessment of Uterus and Fallopian Tubes
Assessment of the uterine contour and the tubal patency is an integral part of the basic infertility evaluation.3This may be achieved by hysterosalpingography (HSG) (Figure 2). An HSG consists of radiographic evaluation of the uterine cavity and fallopian tubes after injection of a radio-opaque medium through the cervical canal. Along with laparoscopic dye pertubation, it can best assess tubal patency: the concordance of HSG with laparoscopic dye pertubation is estimated as close to 90%.11 However, patent fallopian tubes on HSG do not confirm that ovum pickup will occur. For example, women with severe endometriosis may have adherent ovaries in the cul de sac with normal fallopian tubes.
Normal hysterosalpingogram. Reprinted from Fertility and Sterility, Volume 83, Baramki TA, Hysterosalpingography, pages 1595–1606, Copyright 2005, with permission from Elsevier.
Ultrasound evaluation in the follicular phase is used to identify uterine fibroids, polyps, and congenital cavitary anomalies such as a septate uterus. At the same time, information on ovarian volume and antral follicle counts can be obtained, making pelvic ultrasound part of the initial workup for infertility. A complete cavitary assessment, however, necessitates either sonohysterography for conditions such as uterine polyps, submucus leiomyomas, or Asherman’s syndrome (uterine synechiae).12 
Sonohysterography, an office procedure, involves assessing the uterine cavity with ultrasound with concurrent instillation of sterile water. Some practitioners prefer diagnostic office hysteroscopy as it allows direct visualization of the uterine cavity.
The Role of Laparoscopy in the Infertility Evaluation
The role of laparoscopy in the investigation of infertility has changed over the past decade. Whereas laparoscopy used to be part of the basic infertility workup, it is now reserved for selected cases. Given that it allows direct visual examination of the pelvic reproductive anatomy, it is the test of choice to identify otherwise unrecognized peritoneal factors that influence fertility, specifically endometriosis and pelvic adhesions. According to the guidelines of the ASRM, laparoscopy should be performed in women with unexplained infertility or signs and symptoms of endometriosis or in whom reversible adhesive tubal disease is suspected.
Recommendations for the Basic Infertility Evaluation
We recommend that couples have a semen analysis, testing for detection of ovulation (mid luteal progesterone, LH kit), assessment of ovarian reserve, transvaginal ultrasound, and HSG. With this expanded testing, fewer than 15% to 30% of couples will have unexplained infertility.
Treatment of Unexplained Infertility
A diagnosis of unexplained infertility is made after the above-recommended testing fails to reveal any abnormality. The treatment for unexplained infertility is therefore, by definition, empiric because it does not address a specific defect or functional impairment
The principal treatments for unexplained infertility include expectant observation with timed intercourse and lifestyle changes, clomiphene citrate and intrauterine insemination (IUI), controlled ovarian hyperstimulation (COH) with IUI, and IVF.

We present the different treatment options and discuss the advantages and disadvantages of various treatment strategies.
Expectant Management and Lifestyle Changes
Epidemiological studies indicate cigarette smoking, abnormal body mass index (BMI), and excessive caffeine and alcohol consumption reduce fertility in the female partner. The female partner should be counseled to achieve a normal BMI, reduce caffeine intake to no more than 250 mg daily (2 cups of coffee), and reduce alcohol intake to no more than 4 standardized drinks per week.13
The likelihood of pregnancy without treatment among couples with unexplained infertility is less than that of fertile couples but greater than zero.
 It is possible that unexplained infertility represents the lower extreme of the normal distribution of fertility with no defect present. However, it could also be imagined that the routine infertility evaluation misses subtle defects because of imperfect or incomplete testing methods.
Studies of couples with unexplained infertility who are followed without any treatment report a broad variation in cumulative pregnancy rates. A retrospective review of 45 studies by Guzick and colleagues found an average cycle fecundity of 1.3% to 4.1% in the untreated groups, which was lower than most treatment interventions.14
Outcome of expectant management ?? In a recent study, couples with unexplained infertility on a waiting list for IVF in the Netherlands had a 10% to 15% cumulative chance of pregnancy over a 12-month period. As expected, the age of the female partner influenced the pregnancy rate associated with expectant management.15 The currently available evidence suggests that methods prospectively identifying the window of fertility are likely to be more effective for optimally timing intercourse than calendar calculations or BBT.
Although expectant management is associated with the lowest cost, it results in the lowest cycle fecundity rates, and is therefore inferior to the commonly available reproductive techniques outlined below. It may provide an option for a couple with unexplained infertility in whom the female partner is young and the problem of oocyte depletion is not an immediate concern.
Laparoscopy as a Treatment
Whether operative laparoscopy improves pregnancy outcomes in a subject with unexplained or minimal/mild endometriosis is of debate. In 1997, Marcoux and colleagues reported the results of a randomized, controlled trial (RCT) in a population of 341 infertile women 20 to 39 years of age with minimal or mild endometriosis.16During diagnostic laparoscopy the women were randomly assigned to undergo resection or ablation of visible endometriosis or diagnostic laparoscopy only. They were followed for 36 weeks after the laparoscopy or, for those who became pregnant during that interval, for up to 20 weeks of pregnancy.
In the intervention group, 50 of the 170 women became pregnant in the follow-up period, compared with only 29 of 169 in the diagnostic laparoscopy group. The corresponding rates of fecundity were 4.7 and 2.4 per 100 person-months (rate ratio, 1.9; 95% confidence interval, 1.2–3.1). The authors concluded “laparoscopic resection or ablation of minimal and mild endometriosis enhances fecundity in infertile women.”16 Interestingly, a smaller RCT from Italy with a similar study design could not confirm these results. In the study reported by Parazzini,17 the 1-year birth rate in the resection/ablation group was 10 out of 51 women (19.6%), compared with 10 out of 45 women (22.2%) in the no-treatment group. A Cochrane review on the topic published in 2002 concluded that laparoscopic surgery in the treatment of minimal and mild endometriosis may improve pregnancy success rates, but that the “relevant trials have some methodological problems and further research in this area is needed.”18If laparoscopy is performed in a patient with unexplained infertility and minimal/mild endometriosis is identified, we recommend ablation of endometriosis. However, the current literature does not support performing a diagnostic laparoscopy in all patients with unexplained infertility.
IUI
Intrauterine insemination involves the placement of washed sperm into the uterine cavity around the time of ovulation. It can be performed in conjunction with natural ovulation timed with LH kit, ovulation induction using clomiphene citrate, or injectable gonadotropins. Few data exist on the use of IUI without ovarian hyperstimulation (OH). In 1991, Kirby and colleagues reported a RCT of 73 couples with unexplained infertility who were either randomized to IUI or timed intercourse.19 Conceptions occurred in 6 of 145 (4.1%) of the IUI cycles and 3 of 123 (2.4%) of the timed intercourse cycles. A large RCT showed that intracervical insemination alone had lower pregnancy rates per couple compared with IUI alone.20 It has been estimated that 37 cycles of IUI without additional ovarian stimulation would be needed to obtain an additional pregnancy compared with control cycles.2 A recent Cochrane review on this topic confirmed that IUI with ovulation induction increased the live birth rate compared with IUI alone.21 Therefore, IUI without additional treatment with clomiphene citrate or gonadotropins is not routinely performed in couples with unexplained infertility.
COH and IUI
Over the past decades, there has been a marked increase in the use of COH, with or without IUI, in the treatment of unexplained infertility. Both clomiphene citrate and gonadotropins have been used for COH, in combination with IUI or alone. The theoretical rationale for COH in women with a normal ovulatory assessment is that subtle ovulatory defects missed by standard testing may be overcome, and that an increased number of eggs available for fertilization may increase the likelihood of pregnancy. In a similar fashion, introducing washed sperm into the uterine cavity using IUI may increase the density of motile sperm available to ovulated oocytes, which should maximize the chance of fertilization.
Use of clomiphene citrate with timed intercourse in patients with unexplained infertility has been shown to have a small effect on pregnancy rates: combined analysis of the available evidence revealed that 40 cycles with empiric clomiphene citrate therapy were necessary to achieve 1 additional pregnancy.2 Gonadotropin therapy is superior to clomiphene citrate therapy, and both are most effective when combined with IUI. A meta-analysis of 27 studies involving 2939 cycles revealed that the pregnancy rate per cycle was 8% with gonadotropin treatment alone and 18% with gonadotropin treatment combined with IUI.14 The cumulative pregnancy rate rises with the number of attempted COH/IUI cycles; however, there is some evidence suggesting that the number of COH/IUI cycles prior to treatment with IVF should be limited to 3.22 

Aboulghar and colleagues performed an observational prospective study on 594 couples with unexplained infertility to determine the optimum number of COH/IUI cycles. They found that 1 to 3 cycles of COH/IUI resulted in 182 pregnancies, with a cycle fecundity of 16.4% and a cumulative pregnancy rate (PR) of 39.2% (total of 1112 cycles with a mean of 1.9 cycles/patient).22 

Up to 3 further trials of COH/IUI in 91 of these women resulted in only 9 more pregnancies, with a cycle fecundity of 5.6%, significantly lower than that in the first 3 attempts (additional 161 cycles with a mean of 1.8 cycles/patient). The cumulative PR rose to 48.5% by cycle 6, a further increase of only 9.3%.
A historical comparison group with 131 patients with 3 failed cycles of COH/IUI who underwent 1 cycle of IVF at the same center resulted in 48 pregnancies, with a cycle fecundity of 36.6% per cycle, suggesting that patients should be offered IVF if they fail to conceive after 3 trials of COH and IUI.
There are several studies addressing the effect of IUI on 2 consecutive days over single IUI.2325 Available trials on this issue are difficult to interpret because they are not restricted to patients with unexplained infertility, but also included subjects with other types of infertility, such as male factor and cervical factor. Although some studies suggested marginal benefits of double IUI over single, the most recent randomized trial concluded that among patients undergoing COH/IUI, results of single and double IUI do not statistically differ.23 Therefore, double IUI is not routinely offered.
IVF/ICSI
The most expensive, but also most successful treatment of unexplained infertility consists of the spectrum of assisted reproductive technology including IVF, with or without ICSI. IVF is the treatment of choice for unexplained infertility when the less costly, but also less successful treatment modalities outlined above have failed. In the 2006 SART data for unexplained infertility, there were 126,726 completed cycles with 40.4% live birth rate for women younger than 35 years of age and 38.9% for women 35 to 37 years of age.

In addition to offering the highest success rate, IVF also explains infertility in some of these couples. In some IVF programs ICSI is performed in all couples with unexplained infertility (for an undetected fertilization problem), whereas other programs may perform ICSI in 50% of the retrieved oocytes.
Comparison of Different Treatments for Unexplained Infertility
A randomized trial comparing each of these treatment alternatives against one another and a nontreated control group has not been performed. The European Society for Human Reproduction and Embryology (ESHRE) Multicentre Trial reported in 1991 that pregnancy rates per cycle were 15.2% in gonadotropin-only cycles, 27.4% in gonadotropin and IUI cycles, and 25.7% in IVF cycles.26 The pregnancy rate for IVF cycles has since increased with availability of improved and sequential media, embryo micromanipulation, and extended embryo culture. The ASRM Practice Committee published an analysis on the appropriate roles and the cost-effectiveness of the various procedures in the management of unexplained infertility by analysis of previously published data.2,14 There is limited information on the cost-effectiveness of various treatments; however, it appears that there is a correlation between the cost of a treatment modality and its pregnancy rates. Therefore, depending on the individual couple and their particular clinical situation, COH with IUI may be attempted first, with transition to IVF/ICSI if pregnancy is not achieved in a timely manner.
A RCT of clomiphene citrate/IUI versus IVF (Fast Track and Standard Treatment [FASTT] Trial) has recently been completed. In this trial, Reindollar and colleagues27 studied 503 couples assigned to conventional infertility treatment or an accelerated track to IVF. All couples had unexplained infertility and underwent infertility treatment for the first time. Patients were randomized to receive either a conventional treatment regimen of 3 cycles of clomiphene/IUI, 3 cycles of FSH/IUI, and up to 6 cycles of IVF or to receive an accelerated treatment course of 3 cycles of clomiphene citrate/IUI and then up to 6 cycles of IVF. In the conventional arm, 247 couples underwent 646 clomiphene citrate/IUI, 439 FSH/IUI, and 261 IVF treatment cycles; in the accelerated arm, 256 couples received 642 clomiphene citrate and 357 IVF cycles. As of April 2007, 43/232 (18.5%) of the women in the conventional arm became clinically pregnant after clomiphene citrate/IUI, 43/170 (25.3%) after FSH/IUI cycle, and 71/111 (64%) after IVF. In the accelerated arm, 50/242 (20.7%) became pregnant after clomiphene citrate/IUI and 117/171 (68.4%) in an IVF cycle. The median time to pregnancy in the accelerated arm was shorter than the conventional arm. The complete study has not yet been published.
Summary and Conclusions
A thorough but time-efficient investigation of the infertile couple is required prior to a diagnosis of unexplained infertility. Couples should undergo a semen analysis, ovulation testing, assessment of ovarian reserve, and imaging to assess for tubal and uterine factors before a diagnosis of unexplained infertility is made. This workup can be completed within 1 menstrual cycle. In the couples with unexplained infertility, various treatment modalities are available, including expectant management with lifestyle changes, operative laparoscopy, COH (clomiphene citrate or gonadotropins) with IUI, and IVF (with or without ICSI). The optimal treatment strategy needs to be based on individual patient characteristics such as age, treatment efficacy, side-effect profile such as multiple pregnancy, and cost considerations.
Main Points
Couples should undergo a semen analysis, ovulation testing, assessment of ovarian reserve, and imaging to assess for tubal and uterine factors before a diagnosis of unexplained infertility is made.
The principal treatments for unexplained infertility include expectant observation with timed intercourse and lifestyle changes, clomiphene citrate and intrauterine insemination (IUI), controlled ovarian hyperstimulation with IUI, and in vitro fertilization (IVF).
Although expectant management is associated with the lowest cost, it results in the lowest cycle fecundity rates. It may provide an option for a couple with unexplained infertility in whom the female partner is young and the problem of oocyte depletion is not an immediate concern.
The most expensive, but also most successful treatment of unexplained infertility consists of the spectrum of assisted reproductive technology including IVF, with or without intracytoplasmic sperm injection. IVF is the treatment of choice for unexplained infertility when the less costly, but also less successful treatment modalities have failed.
The optimal treatment strategy needs to be based on individual patient characteristics such as age, treatment efficacy, side-effect profile such as multiple pregnancy, and cost considerations.





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