Causes of repeated IUI failures?? If repeated failure in IUI cycles : Then Sperm function tets are mandatory. Functional tests for sperms may be done if there is repeated IUI failure for which no cause could be established including genital Koch's /minimal endometriosis: Such tests are A) Hypo osmotic swelling tests: Sperm fertilizing capability—B) nuclear chromatin decondensation test, C) presence of excessive reactive oxygen species.
Test I:-Hypo-osmotic swelling.
These tests determine the sperm membrane structure and function. The presence of large number of vital (living) , but immotile sperms may be indicative of structural defect in flagellum. Hypo-osmotic Swelling Test offers different information about but sperm membrane function is offered by viability test.
When live spermatozoa are exposed to a solution of low osmolarity 33-80% sperms demonstrate tail swelling. The percentage of sperms, with such curled tails in the treated sample, should be subtracted from the percentage of those that reacted from hypo-osmotic solution.
A normal value is taken as more than 55-60% reacted sperms per sample, and an abnormal value is less than 50% reacted sperms (< 50% coiling). However, this test lacks sufficient critical evaluation to determine its role in evaluation of
Basics: This test is based upon the fact that fluid transport occurs across the intact cell membrane under hypo- osmotic conditions until equilibrium is reached between the inside and outside.HOS values may be selectively altered with certain procedures like: Cryopreservation, Presence of sperm antibodies.
Note: Since some spermatozoa may have curled tails before exposure to hypo-osmotic solution, it is essential to examine the ejaculate before the test. Increase in free radicals, which occur when there is increase in number white blood cells in semen.
.
Excess ROS may be a cause of your IUI failure: ROS are usually washed away with semen preparation in IUI/ART: Reactive oxygen species are metabolites of oxygen and include A) superoxide anion, B) hydrogen peroxide, C) hydroxyl radical and D) nitric oxide.
ROS is produced by both spermatozoa and leucocytes. Increased number of sperms would produce more ROS. When present in excess they can initiate oxidative damage to cellular lipids, proteins and DNA.
Fortunately most cells are equipped with enzymatic or non enzymatic antioxidant systems. The seminal plasma also has these enzymes. Now coming to sperm preparation , while preparing sperms, seminal plasma is removed and the sperms are rendered vulnerable to oxidative attack affecting sperm function.
LikeComment
Comments
Write a comment...
Test I:-Hypo-osmotic swelling.
These tests determine the sperm membrane structure and function. The presence of large number of vital (living) , but immotile sperms may be indicative of structural defect in flagellum. Hypo-osmotic Swelling Test offers different information about but sperm membrane function is offered by viability test.
When live spermatozoa are exposed to a solution of low osmolarity 33-80% sperms demonstrate tail swelling. The percentage of sperms, with such curled tails in the treated sample, should be subtracted from the percentage of those that reacted from hypo-osmotic solution.
A normal value is taken as more than 55-60% reacted sperms per sample, and an abnormal value is less than 50% reacted sperms (< 50% coiling). However, this test lacks sufficient critical evaluation to determine its role in evaluation of
Basics: This test is based upon the fact that fluid transport occurs across the intact cell membrane under hypo- osmotic conditions until equilibrium is reached between the inside and outside.HOS values may be selectively altered with certain procedures like: Cryopreservation, Presence of sperm antibodies.
Note: Since some spermatozoa may have curled tails before exposure to hypo-osmotic solution, it is essential to examine the ejaculate before the test. Increase in free radicals, which occur when there is increase in number white blood cells in semen.
.
Excess ROS may be a cause of your IUI failure: ROS are usually washed away with semen preparation in IUI/ART: Reactive oxygen species are metabolites of oxygen and include A) superoxide anion, B) hydrogen peroxide, C) hydroxyl radical and D) nitric oxide.
ROS is produced by both spermatozoa and leucocytes. Increased number of sperms would produce more ROS. When present in excess they can initiate oxidative damage to cellular lipids, proteins and DNA.
Fortunately most cells are equipped with enzymatic or non enzymatic antioxidant systems. The seminal plasma also has these enzymes. Now coming to sperm preparation , while preparing sperms, seminal plasma is removed and the sperms are rendered vulnerable to oxidative attack affecting sperm function.
LikeComment
Comments
Write a comment...
No comments:
Post a Comment