Friday, 2 October 2020

Genetic Sonogram & NIPT

 

Should a day come in 22 nd century where routine amniocentesis followed by preservation of “Amniotic Stem Cells” for later use if warranted??

Q 1. What is meant by  prenatal  foetal abnormality  diagnosis ? Ans:  Prenatal   diagnosis is the branch of medicine and obstetrics in particular and implies those  studies and some special  techniques   that reveal the normality or the presence of disease in foetus of various   kinds including chromosomal / genetic diseases . All such special techniques   of prenatal   diagnosis   are performed    during pregnancy   and may be direct methods ( invasive)  or indirect methods  (maternal serum markers).  

Q.3: When fetal genetic conditions are warranted? Ans: .Exclusion of fetal genetic abnormalities ( Prenatal diagnosis)  is a standard part of modern obstetric care and there are many indications of such foetal chromosomal analysis . Such are 1) family history of genetic diseases 2) Recurrent foetal loss of uncertain origin 3) Past history of unexplained Rec miscarriages/ abortions and most importantly abnormal serum markers and  abnormal NT scan parameters .

 Q.4: What is the drawbacks of direct tets like CVS amniocentesis?. Ans: Unfortunately, many of the current methods rely on invasive   methods (CVS/amniocentesis) and are associated with an inherent risk of fetal loss. Consequently there has been   a long term   advantage for need    of NIPD(Non Invasive prenatal  diagnois)   .

 What is cffdna test in maternal blood?  Recently an extremely sensitive test for the most   common aneuploidies   and in particular.   Down syndrome  has been  proposed to be performed  on maternal blood.  This test   is based on the count of fragments of specific   chromosomes in maternal   blood  although   not belonging to diagnostic  tests but to probabilistic ones  this test is absolutely the most accurate so far available  with values  around 99.99%   sensitivity and 0.2%   false   positives   . Also   ultrasound  in th second  trimester  of pregnancy      that can  detect  any malformation or fetal abnormality(TIFA )  and fetal   echocardiography   which   analyzes sonographically the fetal heart . Al these tests  carried out on foetus collectively  not only anatomically but also  from the dynamic functional point of view may be  considered   methods of prenatal diagnosis. This technique   however cannot identify genetic   diseases but there  are some markers either in serum or in foetal morphology which may raise an suspicion of some chromosomal / genetic diseases of foetus(genetic sonogram).

 

Role of Molecular genetics ?? Ans:- The  phenomenal developments  in molecular genetics and technological  refinements which    have occurred  over the past  decades have    revolutionized   the area of prenatal genetics  , state of the  art care   commences  with  comprehensive   preconceptional  counseling  .WE cannot perform such tests without the consent of couple as we practice in HIV blood tests  (one may opt out or opt in) .

Conclusion:- Prenatal  diagnosis is now feasible  from the moment   of conception    onward   . Imaging   techniques ( NT Scan parameters & Anomaly Scan )   have allowed non invasive   diagnosis to a great extent but not confirmatory of chromosomal / genetic disorders though such procedures NT scan and Anomaly scan cab exclude anatomical abnormalities and syndromes. However such may fatal or compatible  with life and may of genetic disorders or nongenetic etiology. NIPT on the contrary (minimally invasive techniques )concentrate on sampling   maternal   blood for fetal cells or markers of feto placental metabolism.

It is true that invasive  techniques   have been   rapidly expanding   and becoming safer  comprising   of  A) chorionic   villus  sampling   B) early amniocentesis or C)  midtrimester   amniocentesis  as well as very early  fetoscopy and umbilical vein   sampling .

Advances in prenatal  diagnostic   techniques   allow for   earlier more   rapid and more   effective detection of congenital  disorders  Recent   advances  in non invasive   detection  methods such as fetal    ultrasound    and the isolation   of fetal cells in the maternal circulation   allow the intrauterine  diagnosis  of congenital infections and chromosomal  and Mendelian disorders  as well as   hematologic disorders.

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