Saturday, 17 October 2020

Lactobacillus or Döderlein Bacilli

 White Discharge- A menace for Woman. Leucorrhoea-Persistent long term annoying white discharge with or without pruritus vulvae, may or may not be associated with Urinary symptoms .Possibly 1 in 5 cases of gynae cases seen at our clinic are of such symptoms, so also at OPD.

Lactobacilli are the most abundant bacteria present in normal vaginas of women of child-bearing age (with some exceptions due to race, geographic location and ethnic groups) .

How many of us are aware that Lactobacilli (D Bacilli)  are mainly derived from intestinal microbiota. This symptom of vaginal discharge is strongly suspicious of some common but drug resistant infections as such come to our clinic after  some treatement by one / two GP or even  occasionally by two-three consultants. It is therefore presumable that such cases are some resistant strains of bacteria or fungus and therefore warrant some uncommon investigations which are not always available in all towns(fungal culture) . Leucorrhoea / Whites are therefore --It’s a challenge for the Gynaecologists ll!

Q. 1: Vaginal Ecosystem & Immune system of vaginal mucosa and vaginal fluid is the least discussed topic.

Why and how entry of offending bacteria, parasites and viruses inside the vaginal canal are continuously prevented still remains an enigma to  even the Gnaecologists of repute.

Q.2: What are the normal vaginal Flora???

         Let us talk about most commonly discussed bacteria- i.e. Lacto Bacilli(D. Bacilli) .

         1) There are about 100 species of L B.

         2) They principally produce Lactic Acid as well as Hydrogen Peroxide.

        .

Q. 3 : Besides  Hydrogen  Peroxide and Lac acid there are  the Defensins which maintains ecosystem of vaginal mucosa .

         Defensins: :-Usually neutrophils and macrophages of body excrete this substances to fight against any invading organism. At vaginal fluid  Lact. Bacilli also synthesize such some other antibacterial substances to kill against invading organisms

Q.4: What are Bacteriocins. Who normally liberates   Bacteriocins? It is   by Lact. Bacilli???

         IV) Bacteriocins. These are group of anti-bacterial substances (chemical) which are produced by one strain of bacteria which are harmful against another strain of the same family.

Q.5. How the defense system is normally maintained?

         It is already known that 1) L. Acid, 2)  H2O2, 3) Defensins, & 4) Bacteriocins .-all collectively fight with the  invasive organisms . These four are important both in prevention of overgrowth of pathogenic bact & their proliferation. Additionally one should also think of about pH of vaginal fluid- which is normally   3.5-4.5.à This high pH is key inhibitor in preventing growth of anaerobic organisms. 

Q. 6: Persistent white discharge not responding to your diagnois!!! Please do consider if she oestrogen-deficient clinically because of the fact that the population and work force of L. Bacilli &  Doderlein   bacilli depend much on estrogen.

         If the women concerned is oestrogen deficient her conc. of Doderlein   bacilli, (L. Bacilli) , H2O2  and Lac. Acid   in vagina will be less.

         As such, she  will be a candidate for recurrent  bacterial vaginosis not amenable to the most appropriate antimicrobials as was selected by the clinicians  .

 

Q.7: Where are the sources of normal virginal secretions?

         After attaining adulthood--Vagina is almost always moist (oestrogen effect) . It  comes from transudation from multilayered virginal mucosa . Incidentally Vaginal mucosa do not contain any glands (in contrast to esophagus) to secrete any fluid.

 

Q.8:-What are the normal & usual sources of vaginal secretions?

         Sources are:- Transudates from vaginal  walls, cervical secretions, Endometrial and tubal fluids, secretions from sebaceous glands, sweat glands, Skene glands of ext. urethral glands , Bartholin glands. Any such gland can pour & cause  much-  annoyance to the woman concerned.

 

Q.9:-Qualities of Normal Vaginal Fluid- How it looks and feels like? Ans: White, Thin, flocculent, without any smell/ pruritus. It contains many micro-organisms and their metabolic products that are excreted in the fluid.

         Normally there is a bio-film of normal commensal (Doderlein   bacilli)  in the vaginal mucosaà prevents entry of bacteria into the mucosa.

Reproductive Hormones are the key regulators of amount, character of Discharge.

 

 

 

 

Q. 10 : The history of discovery of Lacto bacilli ?    Ans ; Lactobacilli are the most abundant bacteria present in normal vaginas of women of child-bearing age. In the last decade, there have been quite a lot of scientific advances reported regarding the immunology and pathophysiology of human vaginal microbiota. As a result, physicians have a more comprehensive understanding of the role that lactobacilli exert in the vaginal milieu and their interactions- with vaginal mucosa, pathogenic bacteria and fungi. There have been advances in vaginal microflora, vaginal epithelial cells, immunity, bacterial vaginosis (BV) and candidiasis. Additionally, recent reports regarding

Lactobacilli are the most abundant bacteria present in normal vaginas of women of child-bearing age (with some exceptions due to race, geographic location and ethnic groups) .They are mainly derived from intestinal microbiota. The significance of lactobacilli presence for a healthy vaginal milieu has been recognized ever since Albert S Doderlein discovered a vaginal bacillus that he named Döderlein, in 1892. This bacillus was subsequently renamed as Lactobacillus .

Döderlein claimed that, in normal vaginal secretions, the combination of Döderlein's bacilli and acidity was essential for keeping the vagina free of pathogenic bacteria. This fundamental concept still continues to be utilized today

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