Thursday, 16 May 2019

Tumour markers in the blood before biopsy in cases if supposed cancer.Which marker for which Cancer??

CANCER  MARKERS: What are they?? Suppose you think   that your pt is having cancer somewhere in body and therefore before U insist on some invasive U ask for some markers to strengthen your diagnosis of invasive procedures or say prognosis after definitive therapy Tumour marker is a substance present in or produced by a tumour itself or produced by the host in response to a tumour from normal tissue or to determine the presence of a tumour based on measurement in blood or secretions. It is measured qualitatively or quantitatively by chemical immunological or molecular methods to identify cancer presence . ideally tumour marker should occur only inpatients with malignancy should correlate with stage and response to treatment and should be easily and reproducibly measured. No tumour marker has met this ideal yet. Tumor markers are either polypeptides protein hormones surface antigens cytokines oncogenes or gene products. Baseline levels measured prior to therapeutic intervention and followed later by serial periodic measurements help to predict outcome of therapy. Tumour markers also help in early detection of recurrence relapses and metastasis. However it is the rate of change of the tumour marker level which is more important than its absolute vale . These tests should not be used as a screening test but should be correlated with other clinical criteria.
Clinical Applications- Mass screening , differential diagnosis in symptomatic patients clinical staging estimating tumour volume prognostic indicator of disease progression  detecting recurrence evaluating the success of treatment and monitoring response to therapy.
Recommendations for ordering Tumour Marker tests :
Never rely on the result of a single test: Due to nonspecificity associated with most tumour markers it may become difficult to distinguish between malignant and benign disease on the basis of a single test result. Levels within the normal range do not preclude the presence of cancer nor are elevated results an absolute indication of malignancy . serial testing is recommended because most elevations in benign diseases are transient whereas in malignant diseases the level will remain elevated or rise continuously . Test results should always be interpreted in conjunction with other clinical and laboratory findings
1.       Serial testing should be ordered from the same laboratory using the same assay kit: This is important in order to ensure that changes observed in the tumour marker levels during the monitoring process is caused by a change in tumour volume or other tumour activities and not by laboratory variability because different commercial kits can generate different results.
2.       Tumour marker selected for monitoring recurrence must be elevated before surgery: No tumour marker is 100% sensitive to the detection of a particular type of cancer. Hence the tumour marker selected to detect recurrence must be elevated before surgery. Multiple markers should be measured prior to surgery in order to select the tumour marker showing the highest elevation as the marker for monitoring disease activity.
3.       Consider the half life of the tumour marker when interpreting test result: Estimate the time required for the level determined prior to surgery to decline to normal level based on the known half life of the tumour marker e.g. half life of serum PSA is 3-4 days therefore it will take 30 days for as serum PSA at 50 ng/mL to drop to undetectable levels following  successful surgery . It is preferable to wait one entire month before measuring the tumour marker to assess the success of surgery because this is the time required for the preexisting tumour marker in the serum to decline to lower levels.
4.       Consider how the tumour marker is metabolized from blood circulation : Elevated serum tumour markers are frequently seen in patients  with renal or liver disease depending on whether the tumour  marker is removed through glomerular filtration or metabolized by the liver e.g. serum CEA is often elevated in patients with liver diseases because the impaired liver fails to remove CEA from the blood circulation.
5.       Consider ordering multiple tumour markers to improve sensitivity and specificity for diagnosis: Multiple markers have been used to develop a more specific screening strategy for ovarian cancer. It is found that the use of CA 15.3 and TAG 72 in combination with CA 125 can increase the specificity to distinguish malignant from benign disease. Multiple tumour markers that are complimentary to each other should be selected and not those which run parallel to each other.



MARKER                                                                                              ASSOCIATED CANCERS
Hormones
. hcg                                                                                                       Trophoblastic tumour , non-semino-
                                                                                                                Matous testicular tumour
. Calcitonin                                                                                          Medullary thyroid carcinoma
. Catecholamines
And Metabolites                                                                              Pheochromocytoma

Oncofetal  Antigens
. AFP                                      Hepatocellulr carcinoma non seminomatous germ cell testicular
                                                Tumour

. CEA                                      Carcinomas of colon , pancreas, lung, stomach and breast




Mucins and Glycoproteins

.CA 125                                 Ovarian cancer
.CA 19.9                                Pancreas and colon cancer
.CA 72.4                                Gastric and colon cancer
.CA 15.3                                Breast cancer
.Cyfra 21.1                           Non small cell lung cancer

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