Tuesday, 1 October 2019

Myo inostol : DCI excretion is incresd in PCO : Metformin in PCO when


1-10-19
The task of killing of Jackal, the task of killing a Tiger  are not of  same skill

 How many gynaecologist can assure a PCO woman in 3rd decade of Life that  her  future  Quality of life( in PCO women)  in 4th to  6 th decade of life  will be normal. Let me tell you my dear members that the duty & responsibility of gynaecologits do not end with after achieving one or 2 live births in the florid PCO cases.  Achieving preg is like   killing a jackal but to keep her( your old patient)   healthy upto the decades of eighty with  normolipidaemic normotensive,  average wt , with no endometrial cancer free life with no depression . God QOL(quality of life)  in PCO women as like a challenge with a tiger .
By this time we have to come know that PCO is correlated with raised CRP –->metabolic aberrations often seen in PCO women: Why & how? Ans: Vascular inflammatory   process is reflected by the CRP is the most    reliable    circulating marker of chronic low grade   inflammation in PCOS  . -PCOS is a pro inflammatory state    and emerging   data suggest that chronic low grade   inflammation   supports  the development of  metabolic aberration and ovarian dysfunction . CRP is the most    reliable    circulating marker of chronic low grade   inflammation in PCOS  . Recently    CRP   was found to be a direct promoter of the   atherosclerotic   processes and endothelial cell inflammation   leading to athero thrombosis . CRP has a  direct   role in the vascular  inflammatory   process  stimulating  the release   of inflammatory   cytokines  and increasing   endothelial  expression of cellular  adhesion   molecule  which mediate    leukocyte  migration .
Correlation with level of CRP with probality of heart attack??  Ans;-Findings of a study suggest that increased cardiovascular   risk may be seen in 83.3 % of the PCO women with A) CRP > 2.42  mg /1 .But luckily in those women with B)  CRP  values < 1  mg / 1 are  considered C) low  risk  1-3   mg/ 1 are considered  intermediate  risk and  3-10   mg / 1    are considered high   risk for cardiovascular   disease . 

PCO, insulin resistance à Hyperinsulinaemia, abnormal OGTT  :--Till date  the most accurate    method to diagnose insulin   resistance is the OGTT after 75  g glucose challenge even   in adolescent  women. What is the normal value?? Normal values are the following euglycemia :
1) Fasting 70-100  mg/ dL 2) 60 min after   glucose  administration < 180  mg / dL 3)  120 min after glucose administration < 140 mg/ dL
II. But in case of IGTT :--Impaired  glucose   tolerance is defined  when glucose level is > 140  mg/ dL 2 h after glucose load   but < 200 mg /dL 
III. Diabetes   is defined   when glycemia  is > 200 mg/ dL   2 h after   glucose  load
What is the insulin level?? A) Normoinsulinemia : Fasting  < 10 mUI /mL B) 60 min after glucose administration  < 60 mUI/mL c) 120 min after  glucose   administration , =  10 mUI/mL .Of course   the majority  of PCOS   patients   are not diabetic yet but only insulin resistant . Insulin resistance is defined  when insulin value   1 h after OGTT  is  > 60 mUI/ mL  and / or   its level   is not very   close   to the fasting   insulin  value   after 2 h   post glucose administration.
It has  been suggested  that an OGTT    be performed   every   2 years   for those  with normal   glucose tolerance and annually if IFG  or IGT    is present . Glucose screening  recommendation for PCOS  women are  summarized  .
HOMA index : OGTT is not a very comfortable  method  and it is  also expensive  and time consuming for this   reason the need for a simple way of measuring   insulin resistance   has led to the creation  of a large  number   of insulin sensitivity  indices.  The most    used model  is the HOMA     index.
The homeostatic  model for assessment   of insulin resistance    is a simple and noninvasive   method of estimating    insulin   sensitivity  from the steady  glucose   and insulin  concentrations  measured under  fasting  conditions  . it was calculated   using   the following formula .
Examining scientific  literature studies  are very conflicting  to each other   and a  unanimous opinion  on the effectiveness of insulin   sensitizing  drugs  has not yet been  reached. According  to the ASRM committee  of 2008   insulin   sensitizing  agents   should  be considered in patients    with impaired   glucose  tolerance   and PCOS.
In 2010  AE- PCOS  Society   consensus  treatment   emphasized that   metformin  should be  used in women with PCOS   who have  already   started  lifestyle    treatment      and do not   have improvement in IGT   or in those  who have normal   weight  but still having  .
When   administered to insulin resistant patients these drugs    act to increase  target  tissue  responsiveness in order  to reduce   hyperinsulinemia .
In the past   limited   studies    on the use of Diazoxide acarbose and somatostatin  for PCOS women    were conducted  then thiazolidinediones aroused  more interest   while to  date metformin is the most   worldwide studied insulin  sensitizing agent . Moreover   statins  have also been used to improve   lipid profile  in PCOS women .
Metformin
Despite  there is no   universal  consensus  on metformin benefits in PCOS  in this chapter   all the   beneficial  effects of metformin  therapy  in patients   with PCOS are highlighted.
The positive    effects of  metformin have been   demonstrated in nondiabetic women   with PCOS   and they are   associated   with increased   menstrual cyclicity improved   ovulation     and reduction in circulating   androgen levels.
To date   neither   in Europe  nor in the  United  States   metformin   has been   approved for  the treatment   of insulin   resistance  associated  with PCOS  its use should be restricted to those   patients   with IGT   however it is largely  prescribed as an off label drug .
For   off label  use of any  medication it is extremely  important   to fulfill several criteria   for safe  use :
The condition  should  have health  consequences   significant   enough to   warrant     treatment
The   treatment    should have   demonstrated safety and efficacy
The proposed  treatment   should be  superior   to the presently available   alternatives 
 Mechanism   of Action of metformin:-
Metformin   is a second   generation  biguanide  used  as an  oral antihyperglycemic  agent   and it is  approved  by the US  Food   and Drug   Administration   as treatment   for type ll diabetes  mellitus .
It is   considered an insulin   sensitizing  agent because   it lowers   glucose   levels  without   increasing    insulin secretion but   improving   insulin sensitivity 
Metformin causes
 Increased   peripheral  insulin sensitivity by   activating  glucose   transporters   which  allows      passage of glucose into hepatic  and muscle cells  inhibition of hepatic   glucose production
Reduction of circulating free fatty    acid concentration  which  helps    in reducing    gluconeogenesis .
Metformin  activates  the adenosine monophosphate  activated protein kinase pathway    phosphorylaction of threonine in AMPK   is necessary   for  metformin  action resulting   in decreased  glucose   production  and increased  fatty  acid oxidation in hepatocytes  skeletal muscle   cells   and mouse   ovarian tissue.
Furthermore metformin  inhibits  hepatic   gluconeogenesis  through  an AMP   activated protein    kinase   dependent regulation  of the orphan  nuclear    receptor   small heterodimer partner.
Importantly    the actions of metformin are nor  associated with an increase  in insulin secretion and consequently   with hypoglycemia.
Metformin  affects ovarian   function    in a dual  mode.
Alleviation of systemic  insulin   excess acting  upon the ovary   particularly   on steroidogenesis  and follicular    growth 
Direct  ovarian   effect.
Furthermore   metformin acts at the hypothalamic levels  on AMPK   pathway the latter  is essential in the modulation of LH  secretion
During    the last  two decades   some studies   demonstrated  that metformin  inhibits  androstenedione    and testosterone    production form theca  cells through inhibition  of  the steroidogenic    acute regulatory    protein   and 17 a hydroxylase  expression.
 At the  ovarian   level   hyperandrogenic intra follicular    pattern is improved  by a   decrease   in IGF-1   availability  that has an important  role in  controlling  granulosa   cell aromatase   levels.
It has been  shown that  granulosa cells from  women with  PCOS have higher   levels of FSH  receptor    expression  compared with those   from normal   ovaries .
Metformin reduces  FSH      without     altering   cAMP  levels.  This involves  blocking  activation    of CRE  on promoter  ii of CYP19   via inhibition of pCREB   and possible    disruption  of the formation of the CREB – CRTC  2  co activator complex.  This is   via an  AMPK  independent    mechanism . 
Dosage  and side  effects
Metformin   is available as 500, 850 and 1,000 mg  tablets with a target   dose of  1,500-2,550 mg / day.
Metformin  has a dose dependent    absorption  in humans   and its   bioavailability  is  limited to 50-60 %   because   the amount    available   may result    from pre  systemic    clearance  or binding to the intestinal wall.
Therapeutic regimens of metformin    administration are not well  standardized   and its dose   should probably be adjusted  according   to the patient’s   BMI   and insulin  resistance .
For example it was  demonstrated   that nonobese  women with PCOS  respond better than obese women to metformin    treatment   at a dosage  of 1,500   mg/ day   for 6 months   Nonobese    women in fact  showed a statistically   significant    decrease   in serum androgen    level   and fasting insulin level and also an  improvement  in menstrual  cyclicity    . Moreover   it is    possible   that women    who did not respond   to metformin   1.5 g dose  per day   might show  clinical   changes   if the dose is increased  to 2 g.

Common   side effects are gastrointestinal   such as   diarrhea nausea vomiting   bloating  abdominal  discomfort flatulence and  unpleasant metallic taste   in the mouth.
Lactic   acidosis   and hypoglycemia are very rare.
To reduce these side  effects. It is recommended to start metformin  with a low   dose  and then   gradually  increase  within a period  of  4-6 weeks.
Why Creatinine estimation on 6 monthly basis while on Met RY?: In cases of IGTT renal function may be impaired. As such if a man or woman is on long term met  therapy  then  before initiation of metformin ,serum creatinine estimation seems prudent  thereafter on   6 monthly basis .

.Metformin  may cause vitamin  B12  malabsorption  and so  every   patient   should be  monitored for signs   and symptoms  of vitamin  B 12     deficiency    numbness paresthesia  macroglossia  behavioral     changes   and pernicious anemia.
Metformin   prescription     should be avoided in women   with renal   insufficiency   congestive heart     failure    sepsis   or hepatic   dysfunction .Therefore   testing  of hepatic  and renal    function is  necessary  in advance   of prescription  and thereafter yearly   testing  is indicated.
However   it has been   demonstrated    that metformin use for up to 6 months  dose not adversely affect renal   or liver   function    in  a large   sample  of  PCOS   women even   those with   mildly  abnormal    baseline    hepatic  parameters .The length  of metformin  treatment  in PCOS  patients   is not   standardized but   data present in literature    showed that   after a  long term   metformin treatment   drug suspension  is related   to a quick   reversion  of its beneficial  effect  on peripheral  insulin sensitivity. The    insulin resistance  is associated  with
Abnormally low levels  of DCI  in urine  plasma   and insulin target  tissues  
Vote for MI  :: NOTA to DCI::How many members believe that “ PCO is often associated with excessive MI  urinary  excretion : . Intracellular MI  deficiency   in  insulin sensitive  tissues  In the contrary more recently    Nestler  proposed that in a woman   with PCOS   an initial   genetic   or environmental    insult  causing  insulin    resistance   leads to  a compensatory   hyperinsulinemia. The latter   induces a defect   that increases  renal   clearance of  DCI   and this  lead to a reduction   in circulating   DCI and its   availability     to tissue. The consequence   is an intracellular   deficiency   of DCI  and of DCI – IPG   a mediator of insulin action .Diminished release  of DCI IPG  in response   to stimulation   by insulin    results  in a further   decrease   in insulin  sensitivity  .  Moreover  defective DCI-  IPG  release  in response    to insulin   could be due to a  qualitative    defect in the insulin signaling mechanism  that  activates  DCI IPG  mediator     release   from the membrane   there may  be a primary     defect  in the union  of the insulin   receptor  B unit to the G protein   or a defect   in  G protein activation   of phospholipase.



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