Why preg fails to occur?? Idiopathic
subfertility cases?? Molecular characterization of human sperm progesterone
receptor.
Understanding molecular events during sperm stay in the female
Reproductive tract is important to devise the strategies for
facilitating or inhibiting sperm functions in female reproductive tract.
Studies have therefore been undertaken in this field with the objectives of
characterizing sperm proteins that bind to progesterone, a typical female
hormone and also to investigate whether the expression of such sperm proteins
differ in fertile and infertile men. Our previous studies succeeded in
identifying a membrane bound sperm protein that binds to progesterone, but not
to antiprogestin. Studies carried out further demonstrated for the first time
expression of both conventional and membrane
bound PR in the human
testis. A stage specific expression of conventional PR during spermatogenesis
was also seen. Functional characterization of membrane bound PR demonstrated
that both PK-A and PK-C pathways get activated in human spermatozoa in response
to progesterone. Molecular characterization of progesterone binding sperm
protein showed that antibodies against nuclear PR also recognized a ~55kDa
protein, in sperm and testicular lysates. This protein is embrane bound and binds progesterone
indicating it to 
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