Methodology
of estimation of PRL.
Chemilumincescence
method Microparticle immunoassay.
All samples
exhibiting hyperprolacunaemia-should ideally be SCREENED for amount of
macroprolactins to distinguish between true
hyperprolactinaemia and apparent
hyperprolacunaemia due to mixture of macroprolactin molecules.
There are three types of PRL:-
Native PRL:-ng/mL
PRL after PEG precipitation: which is monomeric PRL which
is biologically active -in this recovery is 99%.What does the word recovery implies?
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Rate of recovery.
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60%
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The interpretation is that the
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Blood sample mostly
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Consist monomeric PRL
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Do rate
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40-60%
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Grey zone
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Sample must contain some amount of
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Macroprolactin & oligomeric
Prolactin.
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Recovery rate
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<40%
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Mostly contains macroPRL
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Gel filtration chromatography: When? For differentiation
of macroprolactin & oligomeric PRL: - A separate method is needed that is gel filtration chromatography.
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