Saturday, 30 November 2019

Recurrent Pregnancy loss -Role of chromosomes.


Recurrent pregnancy loss (RPL) is a devastating reproductive problem affecting approximately 5% of couples trying to conceive. Genetic factors are reviewed in terms of random numerical chromosome errors in miscarriage specimens and carriers of structural chromosome rearrangements that may result in unbalanced chromosome errors in pregnancies.
 In 1975, Boue et al reported that 60% of miscarriages were due to numeric cytogenetic abnormalities, specifically aneuploidies or polyploidies, based on cytogenetic analyses of 1500 miscarriages. They proposed that miscarriage occurred on the basis of natural selection; pregnancies with numeric cytogenetic abnormalities were generally not compatible with life, and therefore ended in miscarriage. Edmonds et al and Wilcox et al,  estimated that 30 to 50% of all pregnancies are lost prior to 6 weeks gestation, and of these, 70% are due to numeric cytogenetic errors..

Pregnancy demise between 6 and 10 weeks of gestation occurs in approximately 15% of clinical pregnancies, of which 50% are due to numeric cytogenetic errors. After 10 weeks of gestation, pregnancy loss is infrequent, estimated at approximately 2 to 3%, of which only 5 to 6% are due to numeric cytogenetic errors. Only 0.6% of term deliveries will have a cytogenetic abnormality, most often trisomy 21, 18, or 13, or a sex chromosome aneuploidy


, genetic factors have been reviewed in terms of random numerical chromosome errors in miscarriage specimens and carriers of structural chromosome rearrangements that may result in unbalanced chromosome errors in pregnancies.
What is skewed X-chromosome inactivation?? Ans:- Recently, research has generated interest in genetic markers for recurrent loss such as skewed X-chromosome inactivation and human leukocyte antigen-G polymorphisms. Assisted reproductive technologies (specifically, preimplantation genetic diagnosis) have been offered to couples with recurrent pregnancy loss; however, more data need to be evaluated before routine use can be advocated. Management of genetic factors in RPL should include therapy based on the highest level of evidence, genetic counseling, and close monitoring of subsequent pregnancies.
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genetic diagnosis
Recurrent pregnancy loss, a devastating reproductive problem, affects approximately 5% of couples trying to conceive. Although much research has sought to determine causation, just a handful of factors have been found to be associated with this reproductive disorder.
Observations on the inefficiency of reproduction prompted researchers to assess the cytogenetics of miscarriages.

In 1975, Boue et al  reported that 60% of miscarriages were due to numeric cytogenetic abnormalities, specifically aneuploidies or polyploidies, based on cytogenetic analyses of 1500 miscarriages. They proposed that miscarriage occurred on the basis of natural selection; pregnancies with numeric cytogenetic abnormalities were generally not compatible with life, and therefore ended in miscarriage.
Additional epidemiological studies have confirmed the original work of Boue et al  .Based on the pioneering works of Edmonds et al[and Wilcox et al. it is estimated that 30 to 50% of all pregnancies are lost prior to 6 weeks gestation, and of these, 70% are due to numeric cytogenetic errors. Pregnancy demise between 6 and 10 weeks of gestation occurs in approximately 15% of clinical pregnancies, of which 50% are due to numeric cytogenetic errors. After 10 weeks of gestation, pregnancy loss is infrequent, estimated at approximately 2 to 3%, of which only 5 to 6% are due to numeric cytogenetic errors. Only 0.6% of term deliveries will have a cytogenetic abnormality, most often trisomy 21, 18, or 13, or a sex chromosome aneuploidy
These statistics are based on studies in the general reproductive population. Human reproduction appears to be an inefficient process due to errors in meiosis or somatic mitosis, which result in a nonviable pregnancy loss due to a cytogenetic abnormality. Whether the frequency of miscarriages with cytogenetic abnormalities is similar or increased in couples with recurrent pregnancy loss remains unanswered at this time. If the risk of recurrence is increased, a role for preimplantation genetic diagnosis may exist. Conversely, if the risk of recurrence is similar to that of the general reproductive population, such technology would be ineffective in improving the live birth rate in couples with reproductive loss.
Numeric and structural cytogenetic errors seen in miscarriages from couples with a history of recurrent pregnancy loss. In addition, literature on pregnancy outcome of translocation carriers, ascertained on the basis of recurrent pregnancy loss, suggest such disorder.  highlighted, along with its limitations. Some of the recent molecular genetic research in the field, such as X-chromosome inactivation and human leukocyte antigen-G (HLA-G) polymorphisms, Given that preimplantation genetic diagnosis is presently being offered in some centers for treatment of recurrent pregnancy loss.


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