Peroxidase stain of semen

 

How to differentiate Pus cells from Round cells (immature sperm cells) ?? What is peroxidase staining?? Ans: The ejaculate contains cells, other than spermatozoa, referred to as round cells. These include germinal line cells sloughed cells from seminiferous tubules, epithelial cells from genitourinary tract, prostate cells, spermatogenic cells and leukocytes. A normal ejaculate should not contain more than 1 x 10 ⁶ round cells/ml. The number of leucocytes should not exceed 1 x 10  ⁶. The concentration of granulocytes in semen can be determined using peroxidase staining and hemocytometer counts.

Round cells that lack polymorphonuclear morphology may be 1) immature germinal cells (spermatocytes, spermatids) 2) exfoliated epithelial cells. These may be degenerating and difficult to identify. To identify them special staining procedures with monoclonal antibodies are used. If round cells are more than 1 million it should be ascertained whether they are leucocytes or spermatids, as leucocytes more than 1 million indicate an infection which may affect fertility. Although CASA system can be used to automatically measure sperm concentration, errors are encountered when there are high and low concentrations, significant agglutinates or large amount of significant debris. The manual count can be obtained with a coefficient of variation of less than 10%.